empty negative control (nc) plasmid  (Shanghai GenePharma)

Journal: American Journal of Cancer Research

Article Title: E74-like ETS transcription factor 1 promotes the progression of pancreatic cancer by regulating doublecortin-like kinase 1/Janus kinase/signal transducer and activator of transcription pathway

doi:

Figure Lengend Snippet: Effects of ELF1 on the proliferation and apoptosis of PC cells. A. The transfection efficiency of ELF1 overexpression plasmid and si-ELF1 was detected by Western blot. B. The effects of ELF1 overexpression or knockdown on the viability of Capan-2 and PANC-1 cells were detected by CCK-8 assay. C. The effects of ELF1 overexpression or knockdown on the proliferation of Capan-2 and PANC-1 cells were detected by EdU assay (scale bars =50 μm). D. The effects of ELF1 overexpression or knockdown on the apoptosis of Capan-2 and PANC-1 cells were detected by flow cytometry. *P < 0.05, **P < 0.01, and ***P < 0.001.

Article Snippet: Cell transfection Empty plasmid (negative control, NC), ELF1 overexpression plasmid (ELF1), DCLK1 overexpression plasmid (DCLK1), small interfering RNA (siRNA) targeting ELF1 (si-ELF1) (si-ELF1-1: sense, 5’-CACUUCAAAUAGGAAUCAAC-3’; anti-sense, 5’-GUUGAUUCCUAUUUGAAGUG-3’; si-ELF1-2: sense, 5’-UCCGACCGAGUCGUCCAUGUA-3’; anti-sense, 5’-UACAUGGACGACUCGGUCGGA-3’), siRNA targeting DCLK1 (si-DCLK1; sense, 5’-GAUCGAUACUUCAAAGGGA-3’; anti-sense, 5’-UCCCUUUGAAGUAUCGAUC-3’), and negative control (si-NC; sense, 5’-UUCUCCGAACGUGUCACGUTT-3’; anti-sense, 5’-ACGUGACACGUUCGGAGAATT-3’) were purchased from GenePharma (Shanghai, China).

Techniques: Transfection, Over Expression, Plasmid Preparation, Western Blot, Knockdown, CCK-8 Assay, EdU Assay, Flow Cytometry

Journal: American Journal of Cancer Research

Article Title: E74-like ETS transcription factor 1 promotes the progression of pancreatic cancer by regulating doublecortin-like kinase 1/Janus kinase/signal transducer and activator of transcription pathway

doi:

Figure Lengend Snippet: ELF1 interferes with cell proliferation and apoptosis by targeting DCLK1. The ELF1 overexpression plasmid and si-DCLK1 were co-transfected into Capan-2 cells, and the si-ELF1 and DCLC1 overexpression plasmids were co-transfected into PANC-1 cells. A. DCLK1 mRNA expression was probed by qRT-PCR after transfection. B, C. The viability of Capan-2 and PANC-1 cells was detected by CCK-8 and EdU assays. D. The apoptosis of Capan-2 and PANC-1 cells was detected by flow cytometry. *P < 0.05, **P < 0.01, and ***P < 0.001.

Article Snippet: Cell transfection Empty plasmid (negative control, NC), ELF1 overexpression plasmid (ELF1), DCLK1 overexpression plasmid (DCLK1), small interfering RNA (siRNA) targeting ELF1 (si-ELF1) (si-ELF1-1: sense, 5’-CACUUCAAAUAGGAAUCAAC-3’; anti-sense, 5’-GUUGAUUCCUAUUUGAAGUG-3’; si-ELF1-2: sense, 5’-UCCGACCGAGUCGUCCAUGUA-3’; anti-sense, 5’-UACAUGGACGACUCGGUCGGA-3’), siRNA targeting DCLK1 (si-DCLK1; sense, 5’-GAUCGAUACUUCAAAGGGA-3’; anti-sense, 5’-UCCCUUUGAAGUAUCGAUC-3’), and negative control (si-NC; sense, 5’-UUCUCCGAACGUGUCACGUTT-3’; anti-sense, 5’-ACGUGACACGUUCGGAGAATT-3’) were purchased from GenePharma (Shanghai, China).

Techniques: Over Expression, Plasmid Preparation, Transfection, Expressing, Quantitative RT-PCR, CCK-8 Assay, Flow Cytometry

Journal: American Journal of Cancer Research

Article Title: The transcription factor TBP promotes hepatocellular carcinoma progression by activating AKT3

doi:

Figure Lengend Snippet: Effects of TBP on the proliferation, migration, invasion and apoptosis of HCC cells. A. The expression of TBP mRNA after transfection of Bel-7402 or HepG2 cells with TBP overexpression plasmid or si-TBP was detected by qRT-PCR. B, C. After transfection, the proliferation of HCC cells was assessed by CCK-8 and EdU experiments. Scale bar = 100 μm. D. After transfection, the apoptosis of HCC cells was assessed by flow cytometry. E, F. After transfection, migration and invasion abilities of HCC cells were assessed by transwell assay. Scale bar = 25 μm. *P < 0.05, **P < 0.01, and ***P < 0.001.

Article Snippet: Cell transfection Empty plasmid (negative control, NC), TBP overexpression plasmid (TBP), AKT3 overexpression plasmid (AKT3), small interfering RNA (siRNA) targeting TBP (si-TBP), siRNA targeting AKT3 (si-AKT3), and negative control siRNA (si-NC) (RiboBio, Shanghai, China) were transfected into Bel-7402 and HepG2 cells with Lipofectamine TM 2000 (Invitrogen, Carlsbad, CA, USA), with the efficiency measured by quantitative real-time PCR (qPCR) 12 h after transfection.

Techniques: Migration, Expressing, Transfection, Over Expression, Plasmid Preparation, Quantitative RT-PCR, CCK-8 Assay, Flow Cytometry, Transwell Assay

Journal: American Journal of Cancer Research

Article Title: The transcription factor TBP promotes hepatocellular carcinoma progression by activating AKT3

doi:

Figure Lengend Snippet: TBP participates in regulating HCC cell proliferation, migration, invasion and apoptosis by modulating AKT3. A. TBP overexpression plasmid and si-AKT3 were co-transfected into Bel-7402 cells, and si-TBP and AKT3 overexpression plasmid were co-transfected into HepG2 cells, and subsequently AKT3 mRNA expression was detected by qRT-PCR. B, C. After transfection, HCC cell proliferation was assessed by CCK-8 assay and EdU assay. D. After transfection, the apoptosis was assessed by flow cytometry. E, F. After transfection, cell migration and invasion abilities were evaluated by transwell experiments. *P < 0.05, **P < 0.01, and ***P < 0.001.

Article Snippet: Cell transfection Empty plasmid (negative control, NC), TBP overexpression plasmid (TBP), AKT3 overexpression plasmid (AKT3), small interfering RNA (siRNA) targeting TBP (si-TBP), siRNA targeting AKT3 (si-AKT3), and negative control siRNA (si-NC) (RiboBio, Shanghai, China) were transfected into Bel-7402 and HepG2 cells with Lipofectamine TM 2000 (Invitrogen, Carlsbad, CA, USA), with the efficiency measured by quantitative real-time PCR (qPCR) 12 h after transfection.

Techniques: Migration, Over Expression, Plasmid Preparation, Transfection, Expressing, Quantitative RT-PCR, CCK-8 Assay, EdU Assay, Flow Cytometry